Using recently established 8 SNU human hepatocellular carcinoma cell lines and Hep 3B cell line, we performed in vitro chemosensitivity test using semiautomated tetrazolium-based colorimetric(MTT) assay. We also measured the MDRI gene expression
of
these cell lines by measuring MDRI RNA level using slot blot analysis. IC50 values(concentration of chemotherapeutic agent which produces 50% growth inhibition of tested cells) were in the range of 1.92~5000 ↑μg/ml for 5-fluorouracil,
0.24~32.00
μg/ml for doxorubicin, 0.16~10.29μg/ml for mitomycin-c,2.00~16.56μg/ml for cisplatin, 1.15~182.32μg/ml for etoposide, and 0.002↓~24↑μg/ml for vin-cristine. Assay area under the curve(A-AUC) at IC50 values showed that only A-AUC of VP-16
was
in a
clinically achievable range for only one of the 9 cell lines. All other drugs were out of the range of clinically achievable AUC.
Three cell lines(SNU-354, SNU-368, SNU-449) were MDR-positive. When these 3 cell lines were compared to MDR-negative cell lines, IC50 value for doxorubicin was significantly higher(p<0.05), suggesting than high expression of MDRI gene is
responsible for
doxorubicin resistance in hepatocellular carcinoma cell lines.
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